The 15-Minute City Quantified Using Mobility Data

Americans travel 7 to 9 miles on average for shopping and recreational activities, which is far longer than the 15-minute (walking) city advocated by ecologically-oriented urban planners. This paper provides a comprehensive analysis of local trip behavior in US cities using GPS data on individual trips from 40 million mobile devices. We define local usage as the share of trips made within 15-minutes walking distance from home, and find that the median US city resident makes only 12% of their daily trips within such a short distance. We find that differences in access to local services can explain eighty percent of the variation in 15-minute usage across metropolitan areas and 74 percent of the variation in usage within metropolitan areas. Differences in historic zoning permissiveness within New York suggest a causal link between access and usage, and that less restrictive zoning rules, such as permitting more mixed-use development, would lead to shorter travel times. Finally, we document a strong correlation between local usage and experienced segregation for poorer, but not richer, urbanites, which suggests that 15-minute cities may also exacerbate the social isolation of marginalized communities

Urban Visual Intelligence: Studying Cities with AI and Street-level Imagery

The visual dimension of cities has been a fundamental subject in urban studies, since the pioneering work of scholars such as Sitte, Lynch, Arnheim, and Jacobs. Several decades later, big data and artificial intelligence (AI) are revolutionizing how people move, sense, and interact with cities. This paper reviews the literature on the appearance and function of cities to illustrate how visual information has been used to understand them. A conceptual framework, Urban Visual Intelligence, is introduced to systematically elaborate on how new image data sources and AI techniques are reshaping the way researchers perceive and measure cities, enabling the study of the physical environment and its interactions with socioeconomic environments at various scales. The paper argues that these new approaches enable researchers to revisit the classic urban theories and themes, and potentially help cities create environments that are more in line with human behaviors and aspirations in the digital age

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Role of urban form in immigrant assimilation

Thesis: M.C.P., Massachusetts Institute of Technology, Department of Urban Studies and Planning, 2016.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Cataloged from student-submitted PDF version of thesis.Includes bibliographical references (pages ).In this thesis I investigate the relationship between the built environment and the residential segregation of immigrants at the building level. I use micro-data that includes the exact address of all the foreign and native population in Barcelona, combined with geometric indicators for urban shape at the block level. Using these data, I construct measures of segregation over time to evaluate the degree to which individuals from different origins, share building space with other immigrants or with the native-born Spaniards. Differences in the built environment appear to have a sizable effect on how immigrants coexist with host communities. The arrival of immigrants to suburban areas is associated with less segregation than other areas. In particular, certain spatial qualities such as open space centrality, coverage and compactness are beneficial in mediating the effect of immigrants on segregation. My results reinforce the idea that a process of suburbanization might have decreased segregation between immigrants and natives, possibly due to native communities being less sensitive to coexisting with immigrant populations in less dense areas.by Arianna Salazar Miranda.M.C.P

Cities and infectious diseases: Assessing the exposure of pedestrians to virus transmission along city streets

As cities resume life in public space, they face the difficult task of retaining outdoor activity while decreasing exposure to airborne viruses, such as the novel coronavirus. Even though the transmission risk is higher in indoor spaces, recent evidence suggests that physical contact outdoors also contributes to an increased virus exposure. Given that streets constitute the largest percentage of public space in cities, there is an increasing need to prioritise their use to minimise transmission risk. However, city officials currently lack the assessment tools to achieve this. This article evaluates the extent to which street segments are associated with spatiotemporal variations of potential exposures of pedestrians to virus transmission. We develop a multi-component risk score that considers both urban form and human activity along streets over time, including (a) an assessment of pedestrian infrastructure according to the average width of pavements, (b) a measure of accessibility for each street based on its position in the street network, (c) an activity exposure score that identifies places along streets where exposure could be higher and (d) an estimate of the number of pedestrians that will pass through each street during weekdays and weekends. We use Amsterdam in the Netherlands as a case study to illustrate how our score could be used to assess the exposure of pedestrians to virus transmission along streets. Our approach can be replicated in other cities facing a similar challenge of bringing life back to the streets while minimising transmission risks.Internet of ThingsHuman-Centred Artificial Intelligenc

Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19

BackgroundWe previously reported that impaired type I IFN activity, due to inborn errors of TLR3- and TLR7-dependent type I interferon (IFN) immunity or to autoantibodies against type I IFN, account for 15-20% of cases of life-threatening COVID-19 in unvaccinated patients. Therefore, the determinants of life-threatening COVID-19 remain to be identified in similar to 80% of cases.MethodsWe report here a genome-wide rare variant burden association analysis in 3269 unvaccinated patients with life-threatening COVID-19, and 1373 unvaccinated SARS-CoV-2-infected individuals without pneumonia. Among the 928 patients tested for autoantibodies against type I IFN, a quarter (234) were positive and were excluded.ResultsNo gene reached genome-wide significance. Under a recessive model, the most significant gene with at-risk variants was TLR7, with an OR of 27.68 (95%CI 1.5-528.7, P=1.1x10(-4)) for biochemically loss-of-function (bLOF) variants. We replicated the enrichment in rare predicted LOF (pLOF) variants at 13 influenza susceptibility loci involved in TLR3-dependent type I IFN immunity (OR=3.70[95%CI 1.3-8.2], P=2.1x10(-4)). This enrichment was further strengthened by (1) adding the recently reported TYK2 and TLR7 COVID-19 loci, particularly under a recessive model (OR=19.65[95%CI 2.1-2635.4], P=3.4x10(-3)), and (2) considering as pLOF branchpoint variants with potentially strong impacts on splicing among the 15 loci (OR=4.40[9%CI 2.3-8.4], P=7.7x10(-8)). Finally, the patients with pLOF/bLOF variants at these 15 loci were significantly younger (mean age [SD]=43.3 [20.3] years) than the other patients (56.0 [17.3] years; P=1.68x10(-5)).ConclusionsRare variants of TLR3- and TLR7-dependent type I IFN immunity genes can underlie life-threatening COVID-19, particularly with recessive inheritance, in patients under 60 years old

Strategies and performance of the CMS silicon tracker alignment during LHC Run 2

The strategies for and the performance of the CMS silicon tracking system alignment during the 2015–2018 data-taking period of the LHC are described. The alignment procedures during and after data taking are explained. Alignment scenarios are also derived for use in the simulation of the detector response. Systematic effects, related to intrinsic symmetries of the alignment task or to external constraints, are discussed and illustrated for different scenarios

Development of the CMS detector for the CERN LHC Run 3

International audienceSince the initial data taking of the CERN LHC, the CMS experiment has undergone substantial upgrades and improvements. This paper discusses the CMS detector as it is configured for the third data-taking period of the CERN LHC, Run 3, which started in 2022. The entire silicon pixel tracking detector was replaced. A new powering system for the superconducting solenoid was installed. The electronics of the hadron calorimeter was upgraded. All the muon electronic systems were upgraded, and new muon detector stations were added, including a gas electron multiplier detector. The precision proton spectrometer was upgraded. The dedicated luminosity detectors and the beam loss monitor were refurbished. Substantial improvements to the trigger, data acquisition, software, and computing systems were also implemented, including a new hybrid CPU/GPU farm for the high-level trigger